Keyboard shortcuts

Press ← or → to navigate between chapters

Press S or / to search in the book

Press ? to show this help

Press Esc to hide this help

Read your results

The default rescue-mode JSON records species_id and subspecies_id at the top level. With --no-anim, the skani-only format records them under assignment. Saved examples retain the mode that produced them; the Markdown and TSV reports provide a common reading format.

Open report.md in the directory you supplied with --report-dir. Start with the final call, reasons and warnings, then examine the supporting comparisons. Use calls.tsv for a batch overview and JSON for a pipeline.

Find the answer

FileUse
report.mdRead the result, warnings and comparison tables.
calls.tsvOne row per assembly with final calls and reasons.
report.jsonFull authoritative report, including versions and provenance.
evidence.tsvSupporting reference comparisons and challenges.

The JSON sent to standard output or --output FILE contains the same report as report.json. TSV and Markdown are derived from it. One input produces an object; a batch produces an array.

The public-genome examples include a species/subspecies assignment with ST286 and a genomovar match, plus an unresolved species call with ST2274. They contain real JSON, TSV and readable reports, with download and reproduction commands.

Separate a call from its nearest reference

calls.tsv reports assigned when the rules support a species, unresolved when a completed run cannot support one, and not_evaluated when no decision was reached. A species can be assigned while subspecies or genomovar remains empty. Withheld assignment fields are null in JSON and empty in the TSV.

A nearest-reference name can still appear beside an unresolved result. Read it as comparison evidence, not as the assigned name. Reasons explain the withholding. Execution errors are separate: check execution_status, execution_stage and execution_error in the TSV, or execution in JSON. Some later-stage failures retain independently supported parent calls; inspect each rank and the failure rather than treating the whole row as a biological negative.

Read the warnings

A genomovar match means compatibility with the supplied definition. It cannot prove that the query’s source group is represented: an unrepresented group can match a neighbour. Overlapping compatible groups are withheld; one-member groups carry a sparse-reference warning. Genomovar matches explains these limits.

ANI and aligned fraction are percentages. An aligned fraction of 0.8 means 0.8%, while 80 means 80%. Missing comparisons are not zero ANI or evidence against an unmeasured rival. A clean report does not certify assembly purity.

For an uncertain call, follow boundary review. The report reference preserves exact field names, method-specific measurements, competitor completeness and version fields. The candidate ANIm workflow retains not_yet_validated in its output; this documentation does not change that status.