LRGE 1.0.0: better genome size estimates from long reads
LRGE (pronounced “large”) estimates genome size directly from long read sequencing data, without assembling the reads into a genome first. We published the paper in 2025 [1], and since then I’ve been working on cases where its estimates go wrong. Those changes are now available in version 1.0.0. The biggest changes are depth normalisation and more selective filtering of internal matches caused by repeats. Getting those working also led to improvements in runtime and memory use, and uncovered a bug that had been affecting every PacBio estimate. ...