Unresolved genome: ECC-219, ST2274
GCA_043966805.1 is a public chromosome assembly labelled Enterobacter sp. ECC-219. It is the second row in the batch report.
| Result | Value |
|---|---|
| Species | Unresolved |
| Subspecies / genomovar | Withheld |
| MLST | ST2274, exact_profile_match |
| Closest species reference | E. nematophilus type anchor, GCA_026344075.1 |
| skani ANI | 94.83% |
| skani aligned fraction | 82.65% query; 88.26% reference |
| Reciprocal ANIm | 95.02965% and 95.03431% |
| Lowest ANIm aligned fraction | 84.94% |
| Reason | ANIm_no_strict_witness |
The genome falls in the range where the tool runs ANIm. That comparison finishes successfully, but neither orientation exceeds the required 96% ANI. The species stays unresolved despite adequate aligned fraction. Empty species fields here are intentional, not missing output or an execution failure.
MLST still finds the complete seven-locus profile:
| dnaA | fusA | gyrB | leuS | pyrG | rplB | rpoB |
|---|---|---|---|---|---|---|
| 590 | 263 | 415 | 493 | 302 | 170 | 101 |
The full JSON contains mlst.sequence_type: "2274" alongside species_name: null. An ST is an allele-profile identifier; it does not establish species membership. Do not copy nematophilus from the nearest-reference table into the assigned-species field.
To investigate further, follow boundary review. Keep the original automatic report and record any subsequent expert interpretation separately.
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